|
KMID : 0545120060160121990
|
|
Journal of Microbiology and Biotechnology
2006 Volume.16 No. 12 p.1990 ~ p.1994
|
|
|
Proteome Analysis of Waito-c Rice Seedlings Treated with Culture Fluid of Gibberellin-producing Fungus, Fusarium proliferatum KGL0401
|
|
Rim Soon-Ok
Lee Jin-Hyung
Hwang Seon-Kap
Suh Seok-Jong
Lee Jin-Man
Rhee In-Koo
Kim Jong-Guk
|
|
|
Abstract
|
|
|
|
Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited a high GA productivity. A gas chromatography-mass spectrometry (GC-MS) analysis of extracts of the culture fluid of F proliferatum KGL0401 also revealed the presence of , , , , , and . Therefore, the present study conducted a proteome analysis of waito-c rice treated with the culture fluid of the isolated F proliferatum KGL0401 to identify the protein expression triggered by the GA-containing culture fluid. The results revealed the overexpression of 180 protein spots in the sample treated with the culture fluid. Among them, 75 induced proteins were selected and analyzed by MALDI-TOF (matrix-assisted laser desorption-iorrization time-of-flight) mass spectrometry, followed by database searching, and 51 proteins were identified.
|
|
|
KEYWORD
|
|
|
Gibberellin, Fusarium proliferatum KGL0401, matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, two-dimensional polyacrylamide gel electrophoresis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|